Anti-DDX58 Monoclonal Antibody

Product Name

Anti-DDX58 Monoclonal Antibody

View all RIG-I Antibodies

SKU/Catalog Number

M00244

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DDX58 Monoclonal Antibody catalog # M00244. Tested in WB, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX58 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00244)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEA-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DDX58 Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00244 is reactive to DDX58 in Human

Applications

M00244 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

106.6kDa

Background of RIG-I

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IP 1:50

Validation Images & Assay Conditions

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Western blot analysis of DDX58 expression in Jurkat cell lysate.

Gene/Protein Information For DDX58 (Source: Uniprot.org, NCBI)

Alternative Names

DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DKFZp686N19181; EC 3.6.4.13; FLJ13599; probable ATP-dependent RNA helicase DDX58; retinoic acid inducible gene I; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; RIG-1; RIGI; RIG-I; RIG-IDKFZp434J1111; RNA helicase RIG-I DDX58 RIG-I, RIG1, RIGI, RLR-1, SGMRT2 DExD/H-box helicase 58 antiviral innate immune response receptor RIG-I|DEAD (Asp-Glu-Ala-Asp) box polypeptide 58|DEAD box protein 58|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RNA helicase RIG-I|probable ATP-dependent RNA helicase DDX58|retinoic acid-inducible gene 1 protein|retinoic acid-inducible gene I protein

For more info on DDX58, check out the DDX58 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDX58: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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